全文获取类型
收费全文 | 920篇 |
免费 | 95篇 |
出版年
2023年 | 2篇 |
2021年 | 5篇 |
2020年 | 9篇 |
2019年 | 18篇 |
2018年 | 18篇 |
2017年 | 15篇 |
2016年 | 24篇 |
2015年 | 29篇 |
2014年 | 36篇 |
2013年 | 45篇 |
2012年 | 61篇 |
2011年 | 64篇 |
2010年 | 36篇 |
2009年 | 34篇 |
2008年 | 45篇 |
2007年 | 58篇 |
2006年 | 62篇 |
2005年 | 57篇 |
2004年 | 56篇 |
2003年 | 40篇 |
2002年 | 51篇 |
2001年 | 23篇 |
2000年 | 24篇 |
1999年 | 23篇 |
1998年 | 15篇 |
1997年 | 10篇 |
1996年 | 7篇 |
1995年 | 12篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 17篇 |
1991年 | 18篇 |
1990年 | 11篇 |
1989年 | 6篇 |
1988年 | 9篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1976年 | 6篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 5篇 |
1971年 | 2篇 |
1929年 | 1篇 |
1928年 | 2篇 |
排序方式: 共有1015条查询结果,搜索用时 15 毫秒
991.
992.
Human-induced processes are altering habitats at an unprecedented rate and scale. This has changed the biodiversity and biomass in many areas, but also led to phenotypic and genetic alterations of populations. Here we investigated the effects of the ongoing eutrophication in the Baltic Sea on the reproductive success of threespine stickleback males Gasterosteus aculeatus , through effects on reproductive behaviour and parenting ability. We allowed males to complete breeding cycles in a competitive setting under increased macro algae cover or increased turbidity caused by phytoplankton growth. Both environmental factors improved the parenting ability of the males and enhanced reproductive output. Increased alga growth and turbidity reduced aggressive interactions between males during the parental phase, probably due to reduced visibility, which slowed down a deterioration of condition. This increased the reproductive lifespan of the males and enabled them to complete more breeding cycles, as found when males were allowed to complete as many breeding cycles as they could under increased algae cover. In addition, increased turbidity improved oxygen conditions, which enhanced hatching success and reduced the need for vigorous fanning behaviour. Increased turbidity, however, relaxed selection on male size. Together with earlier results on relaxed sexual selection under changed environmental conditions, this suggests that the effect of eutrophication on stickleback populations is complex. It increases the reproductive output of populations, since more individuals are spawning within eutrophicated areas and their hatching success is increased, but it relaxes sexual and natural selection at the reproductive stage. Whether this will shift selection and population regulation to other life stages, such as the juvenile stage, deserves further investigations. 相似文献
993.
Cecilia Orellana-Coca Ulrika T rnvall Dietlind Adlercreutz Bo Mattiasson Rajni Hatti-Kaul 《Biocatalysis and Biotransformation》2005,23(6):431-437
Chemo-enzymatic epoxidation of oleic acid (OA) and its methyl ester has been performed using hydrogen peroxide and immobilized lipase from Candida antarctica (Novozym® 435). The purpose of the study was to characterize the reaction under solvent-free conditions. The reaction temperature had a significant impact on epoxidation of OA. At lower temperatures, the substrate conversion was hindered by the formation of solid epoxystearic acid product. Nearly 90% conversion of OA to the epoxide product was obtained after 6 h at 50°C. Longer reaction times at 40°C and above resulted in by-product formation and eventually lowered the product yield. In contrast, the reaction with methyl oleate (MO) was less influenced by temperature. Almost complete epoxidation was achieved at 40-60°C; the higher the temperature the shorter was the reaction time. The main epoxidation product obtained was epoxystearic acid methyl ester (EME), and the remaining was epoxystearic acid (EA) formed by the hydrolytic action of the lipase. Recycling of the lipase for epoxidation of MO at 50°C indicated that the immobilized enzyme was prone to activity loss. 相似文献
994.
M. Beier 《Australian Journal of Entomology》1971,10(4):233-234
i
Pseudotyrannochthonius giganteus sp. n. is described from Calgadup Cave near Augusta, Western Australia. 相似文献
Pseudotyrannochthonius giganteus sp. n. is described from Calgadup Cave near Augusta, Western Australia. 相似文献
995.
996.
J E Nielsen L Beier D Otzen T V Borchert H B Frantzen K V Andersen A Svendsen 《European journal of biochemistry》1999,264(3):816-824
The importance of electrostatics in catalysis has been emphasized in the literature for a large number of enzymes. We examined this hypothesis for the Bacillus licheniformis alpha-amylase by constructing site-directed mutants that were predicted to change the pKa values of the catalytic residues and thus change the pH-activity profile of the enzyme. To change the pKa of the catalytic residues in the active site, we constructed mutations that altered the hydrogen bonding network, mutations that changed the solvent accessibility, and mutations that altered the net charge of the molecule. The results show that changing the hydrogen bonding network near an active site residue or changing the solvent accessibility of an active site residue will very likely result in an enzyme with drastically reduced activity. The differences in the pH-activity profiles for these mutants were modest. pH-activity profiles of mutants which change the net charge on the molecule were significantly different from the wild-type pH-activity profile. The differences were, however, difficult to correlate with the electrostatic field changes calculated. In several cases we observed that pH-activity profiles shifted in the opposite direction compared to the shift predicted from electrostatic calculations. This strongly suggests that electrostatic effects cannot be solely responsible for the pH-activity profile of the B. licheniformis alpha-amylase. 相似文献
997.
998.
Akira Yoshinari Takuya Hosokawa Marcel Pascal Beier Keishi Oshima Yuka Ogino Chiaki Hori Taichi E Takasuka Yoichiro Fukao Toru Fujiwara Junpei Takano 《The Plant cell》2021,33(2):420
Plants take up and translocate nutrients through transporters. In Arabidopsis thaliana, the borate exporter BOR1 acts as a key transporter under boron (B) limitation in the soil. Upon sufficient-B supply, BOR1 undergoes ubiquitination and is transported to the vacuole for degradation, to avoid overaccumulation of B. However, the mechanisms underlying B-sensing and ubiquitination of BOR1 are unknown. In this study, we confirmed the lysine-590 residue in the C-terminal cytosolic region of BOR1 as the direct ubiquitination site and showed that BOR1 undergoes K63-linked polyubiquitination. A forward genetic screen identified that amino acid residues located in vicinity of the substrate-binding pocket of BOR1 are essential for the vacuolar sorting. BOR1 variants that lack B-transport activity showed a significant reduction of polyubiquitination and subsequent vacuolar sorting. Coexpression of wild-type (WT) and a transport-defective variant of BOR1 in the same cells showed degradation of the WT but not the variant upon sufficient-B supply. These findings suggest that polyubiquitination of BOR1 relies on its conformational transition during the transport cycle. We propose a model in which BOR1, as a B transceptor, directly senses the B concentration and promotes its own polyubiquitination and vacuolar sorting for quick and precise maintenance of B homeostasis.The borate transporter BOR1 senses the boron concentration through its borate transport activity for K63-linked polyubiquitination and subsequent degradation. 相似文献
999.
Movement of Aedes aegypti following a sugar meal and its implication in the development of control strategies in Durán,Ecuador 下载免费PDF全文
Whitney A. Qualls Diana P. Naranjo Miguel Angel Subía Giovanni Ramon Varsovia Cevallos Isabel Grijalva Eduardo Gómez Kristopher L. Arheart Douglas O. Fuller John C. Beier 《Journal of vector ecology》2016,41(2):224-231
We evaluated how the presence of sugar sources impacted the distribution of Aedes aegypti in different habitats in Durán , Ecuador. Land cover and normalized difference vegetation index maps were used to guide a random point sampling routine to select study grids (30 m × 30 m) in low vegetation (LV) and high vegetation (HV). Five individual plants, at one home in the LV and HV grid, were treated with a different colored, non‐attractive, 60% sucrose solution to determine mosquito feeding and movement. Sugar alone is not attractive to mosquitoes, so spraying vegetation with a dyed sugar solution can be used for visual determination of sugar feeding. Outdoor collections using BG sentinel traps and indoor collections using aspirators were conducted at the treatment home and with collection points at 20, 40, and 60 m surrounding the treatment home for three consecutive days. A total of 3,245 mosquitoes in two genera, Aedes and Culex, was collected. The proportion of stained Ae. aegypti females was 56.8% (510/898) and 0% for males. For Culex, 63.9% (248/388) females and 36.1% (140/388) males were collected stained. Aedes aegypti and Culex spp. were found up to 60 m stained in both LV and HV grids. Significantly more stained females Ae. aegypti were found inside homes compared to females and males of Culex spp. in both habitats. This study identifies that outdoor sugar feeding is a common behavior of Ae. aegypti and can be targeted as a control strategy in urban habitats in Latin America. 相似文献
1000.
Louise Grahnemo Annica Andersson Merja Nurkkala-Karlsson Alexandra Stubelius Marie K. Lagerquist Mattias N. D. Svensson Claes Ohlsson Hans Carlsten Ulrika Islander 《Arthritis research & therapy》2015,17(1)
IntroductionPostmenopausal women with rheumatoid arthritis (RA) have increased risk of developing osteoporosis due to chronic inflammation and estrogen deprivation. Collagen antibody-induced arthritis (CAIA), an experimental polyarthritis model representing the effector phase of arthritis, is mainly mediated by the innate immune system. Compared to the widely used collagen-induced arthritis model, CAIA is conveniently short and can be used in C57BL/6 mice, enabling studies with knock-out mice. However, the impact on bone of the CAIA model in C57BL/6 mice has not previously been studied. Therefore, the aim of this study was to determine if CAIA can be used to study postmenopausal arthritis-induced osteoporosis.MethodsCAIA was induced by administration of collagen-type II antibodies and lipopolysaccharide to ovariectomized female C57BL/6J mice. Control mice received lipopolysaccharide, but no antibodies. Nine days later, femurs were collected for high-resolution micro-CT and histomorphometry. Serum was used to assess cartilage breakdown and levels of complement. Frequencies of immune cell subsets from bone marrow and lymph nodes were analyzed by flow cytometery.ResultsTrabecular bone mass was decreased and associated with increased number of osteoclasts per bone surface in the CAIA model. Also, the frequency of interleukin-17+ cells in lymph nodes was increased in CAIA.ConclusionThe present study show that CAIA, a short reproducible arthritis model that is compatible with C57BL/6 mice, is associated with increased number of osteoclasts and trabecular bone loss. 相似文献